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fluorescent probe gfp lc3 rfp lc3δg  (Addgene inc)


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    Addgene inc fluorescent probe gfp lc3 rfp lc3δg
    A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
    Fluorescent Probe Gfp Lc3 Rfp Lc3δg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 73 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/gfp+lc3+rfp+probe/pMRX-IP-GFP-LC3-RFP+(Plasmid+%2384573)/bio_rxiv__64898__2026__03__09__709824-42-9-22
    Average 93 stars, based on 73 article reviews
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    1) Product Images from "Physiological cerebrospinal fluid like medium reveals autophagy dependency of leukaemia in the central nervous system"

    Article Title: Physiological cerebrospinal fluid like medium reveals autophagy dependency of leukaemia in the central nervous system

    Journal: bioRxiv

    doi: 10.64898/2026.03.09.709824

    A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
    Figure Legend Snippet: A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).

    Techniques Used: Fluorescence, Expressing, Cell Culture, Western Blot, Isolation, Plasmid Preparation, Control, Knock-Out, Comparison

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    Article Title: A Stress-Induced PI3P Complex Controls Cell Autophagy in Erythroid Precursors
    Article Snippet: .. EPCs were retrovirally infected to express 754 the GFP-LC3-RFP probe (Addgene #84573). ..



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    A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
    Fluorescent Probe Gfp Lc3 Rfp Lc3δg, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    gfp lc3 rfp probe  (Addgene inc)
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    A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
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    A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
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    A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
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    gfp lc3 rfp lc3δg probe  (Addgene inc)
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    A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) <t>cells</t> <t>expressing</t> <t>GFP-LC3-RFP-LC3ΔG</t> autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).
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    A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).

    Journal: bioRxiv

    Article Title: Physiological cerebrospinal fluid like medium reveals autophagy dependency of leukaemia in the central nervous system

    doi: 10.64898/2026.03.09.709824

    Figure Lengend Snippet: A, Schematic diagram of autophagy pathway and the mechanism of action of bafilomycin A1 to inhibit autophagy. B, C, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM (C) and REH (D) cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h with or without 50 nM of bafilomycin A1 treatment (n=7 independent experiments). D, Immunoblot analysis showing changes in autophagy markers in human CD19 + (huCD19 + ) SEM cells isolated from the bone marrow (BM) and the brain (CNS) (n=2 biological replicates). E, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) with or without 1 μM of MRT403 treatment (n=3 independent experiments). F, Percentage of apoptosis in SEM cells cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h in normoxia (21% O 2 ) or hypoxia (1% O 2 ) with or without 50 nM of bafilomycin A1 treatment (n=3 independent experiments). G, Immunoblot analysis showing deletion of ULK1 and changes in autophagy markers in SEM cells cultured in RPMI. H, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM vector control (CTRL) and ULK1 knockout (KO) single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). I, Immunoblot analysis showing deletion of ATG7 and changes in autophagy markers in SEM cells cultured in RPMI. J, Relative GFP/RFP ratio of mean fluorescence intensity (MFI) in SEM CTRL and ATG7 KO single clone 1 and 2 cells expressing GFP-LC3-RFP-LC3ΔG autophagic flux probe cultured in RPMI, Plasmax (PL), and CSFmax (CSF) for 24 h (n=3 independent experiments). Data are shown as the mean ± s.e.m. P-values were calculated with a repeated measure one-way ANOVA with Tukey’s multiple comparison test (B, C, E, F, H, J).

    Article Snippet: Similarly, to generate stable ALL cell lines expressing the fluorescent probe GFP-LC3-RFP-LC3ΔG we used PMRX-IP-GFP-LC3-RFP which was a gift from Noboru Mizushima (Addgene Plasmid #84573) [ ].

    Techniques: Fluorescence, Expressing, Cell Culture, Western Blot, Isolation, Plasmid Preparation, Control, Knock-Out, Comparison